Integrated VHP Space Sterilization System: Application Case Study in a P3 Laboratory

In the bio-pharmaceutical field, ensuring laboratory bio-safety is paramount. Pulike Biological Engineering Co., Ltd., as an industry leader, is committed to providing high-quality products and services for animal health. With its exceptional expertise in clean environment equipment and sterilization technology, TKSAGE (Shenzhen) Technology Group Co., Ltd. developed a professional VHP HVAC sterilization system solution for Pulike's Biosafety Level 3 (BSL-3) laboratory.

The vaporized hydrogen peroxide (VHP) concentration typically requires achieving 400-800 ppm with a maintained duration of 30 minutes, monitored in real time via electrochemical sensors. Optimal sterilization efficiency is achieved when relative humidity is maintained at 30-40%, regulated through an integrated temperature and humidity control system. The suitable temperature range generally falls between 25-35°C, monitored using infrared thermal imaging instruments.

Pulike Biological has extensive experience in the R&D and production of animal vaccines. Its BSL-3 laboratory undertakes critical research tasks, demanding extremely high standards of environmental safety and sterility. Any microbial contamination could compromise experimental results and pose serious bio-safety risks. Therefore, a highly efficient and reliable sterilization system was essential to ensure the laboratory consistently meets stringent standards.

TKSAGE tailored a Vaporized Hydrogen Peroxide (VHP) HVAC sterilization system to address Pulike Biological's specific needs.

Through TKSAGE's VHP HVAC sterilization system, Pulike Biological's BSL-3 laboratory achieved significant enhancements in bio-safety protection. The highly effective sterilization process provided a solid foundation for experimental accuracy and reliability, supporting Pulike Biological in achieving better outcomes in animal vaccine development and disease research.

This collaboration not only demonstrated TKSAGE's professional capability in delivering integrated clean/sterilization equipment solutions but also set a benchmark for bio-safety assurance in the bio-pharmaceutical industry.

Vaccine production demands extremely stringent aseptic environmental conditions in production areas. As a critical institution for COVID-19 vaccine manufacturing, Kunming Institute of Biology (KIB) required a highly efficient and reliable sterilization system to ensure the safety of its production environment. Leveraging its extensive expertise in clean environment equipment and sterilization technologies, TKSAGE Group developed a tailored Vaporized Hydrogen Peroxide (VHP) HVAC Sterilization System solution for KIB.

Through the VHP HVAC Sterilization System provided by TKSAGE Group, the P3 laboratory production area at Kunming Institute of Biology will be equipped with a highly efficient and reliable sterilization system. This offers robust environmental assurance for vaccine production and facilitates the smooth progress of manufacturing operations.

As a pivotal research platform dedicated to cutting-edge scientific exploration and key technological breakthroughs, Chongqing Jinfeng Laboratory imposes exceptionally high requirements for sterility and cleanliness in its experimental environment. Traditional sterilization methods have increasingly revealed limitations when addressing the laboratory’s complex environment and frequent sterilization needs, including issues such as incomplete sterilization, chemical residue, and potential damage to sensitive equipment. These challenges hinder the laboratory’s ability to meet its escalating demands for high-standard scientific research.

Leveraging its profound expertise and innovative capabilities in clean environment equipment and sterilization technology, TKSAGE Group designed a customized Vaporized Hydrogen Peroxide (VHP) HVAC Sterilization System solution for Chongqing Jinfeng Laboratory. This tailored system aims to establish a safe, efficient, and reliable sterile experimental environment, thereby strongly facilitating the smooth progress of the laboratory’s research activities.

With professional technical support, high-quality products, and comprehensive services, TKSAGE Group will ensure the successful implementation of the VHP HVAC Sterilization System at Chongqing Jinfeng Laboratory. This initiative will assist the laboratory in building a world-class sterile research environment, providing solid support for scientific innovation in fields such as life sciences and biomedicine.

TKSAGE Group specializes in delivering integrated clean/sterilization equipment solutions for laboratories and biopharmaceutical applications. For the Biosafety Level 3 (P3) Laboratory at Enshi Center for Disease Control (CDC) in Hubei Province, we developed a customized Vaporized Hydrogen Peroxide (VHP) HVAC Sterilization System.

The system comprises a VHP generator, concentration control system, temperature/humidity regulation modules, ventilation equipment, and pipeline distribution networks. Based on the specific layout and HVAC configuration of the Enshi CDC P3 Laboratory, the VHP generator was installed in a dedicated equipment room adjacent to the AHU machinery space. Stainless steel pipelines connect the generator to the HVAC supply ducts, ensuring uniform distribution of VHP vapor throughout the entire air handling system.

Appendix: Application Parameters of VHP HVAC Sterilization System

The vaporized hydrogen peroxide (VHP) concentration typically requires achieving 400-800 ppm with a maintained duration of 30 minutes, monitored in real time via electrochemical sensors. Optimal sterilization efficiency is achieved when relative humidity is maintained at 30-40%, regulated through an integrated temperature and humidity control system. The suitable temperature range generally falls between 25-35°C, monitored using infrared thermal imaging instruments.

Aerosol distribution uniformity necessitates multi-point injection devices to ensure even vapor diffusion within HVAC ducts, with spatial concentration deviation controlled within ≤15%. After sterilization, a catalytic decomposition module activates to break down residual H₂O₂ into water and oxygen, reducing concentration below 1 ppm to comply with OSHA safety limits. The system demonstrates log6 reduction efficacy, inactivating 99.999% of microorganisms including bacterial spores, viruses, and fungi.

Cleanliness requirements vary across applications: pharmaceutical industries require Grade A (ISO Class 5) standards with ≤1 CFU/4 hours for settling bacteria post-sterilization; medical facilities demand ≥4-log reduction (99.99%); biosafety Level 3 (BSL-3) laboratories require full six-surface sterilization coverage with leak rates <0.01%.